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KMID : 0369820140440060423
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Jorunal of Korean Pharmaceutical Sciences
2014 Volume.44 No. 6 p.423 ~ p.430
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Evaluation of chaperone ability of S. rosmarinus against protein aggregation
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Heidari Abbas
Ghahghaei Arezou
Valizadeh Jafar
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Abstract
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Protein aggregation occurs via a process in which unnatural molecules connect together and create soluble small oligomers or insoluble aggregations. The aggregation of protein depends on resistance conformation and the colloidal characteristics of proteins. Finding proper ways to stabilize or prevent of protein aggregation could be important for the control of such diseases as Alzheimer’s and Parkinson’s. Seidlitzia rosmarinus (S. rosmarinus) extract has antioxidant compounds which can cause an increase in protein resistance and prevent protein aggregation. The aim of this study is to assess the chaperone effects of compounds in S. rosmarinus extract on protein aggregation. In this research, the chaperone property of S. rosmarinus extract was evaluated on ovotransferin, insulin, and α-lactalbumin aggregation, using visible light spectroscopy, florescence and circular dichroism spectroscopy. The results indicate that the extract of S. rosmarinus could prevent protein aggregation in a concentration-dependent manner. The protective effect of S. rosmarinus, however, differed among the three proteins due to their different hydrophobic surface areas.
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KEYWORD
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S. rosmarinus, Chaperone, Aggregation
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